1281 From in vitro modelling to in vivo characterization of solar lentigines for cosmetics evaluation

Over the past 30 years, CHANEL Research has conducted an ambitious program of clinical studies worldwide. As part this plan, we have analyzed thousands women's faces and studied dynamics aging its visible signs such as sagging, wrinkles spots, also known solar lentigines (SL). SL are a major skin concern regardless diversity types. They play important role in perception age therefore constitute challenge for Research. To better characterize molecular mechanisms underlying formation, developed world’s first vitro 3D full-thickness spot model based on unique high-resolution patterned bioprinting approach. This model, including functional individualized hyper pigmentary zone surrounded by defined perilesional zone, was used predictive to evaluate effectiveness our active ingredient AI3401 prevention correction appearance. For vivo approach, LC-OCT, optical technique allowing imaging facial with micrometric cellular resolution. We described different types structures at location classified them into five categories according their histological aspects. were able demonstrate vivo, that cosmetic formula containing ingredient, effective reducing amount melanin treated thus confirmed predictivity new SL. strong innovative strategy, ranging from modelling characterization brightening products cosmetics ingredients prevent correct